
At a Glance
Four facts to hold on to.
Fixers
Alfalfa, chickpea, clover.
Helper
Rhizobium bacteria in root nodules.
Enzyme
Nitrogenase.
UPSC 2022
Answer: 1, 3 and 4 only.
In this lesson
What Nitrogen Fixation Means
Why plants cannot use air nitrogen
- Air is about 78% nitrogen gas (N₂).
- Its two atoms share a very strong bond.
- Plants cannot break it with their own enzymes.
- They absorb nitrogen mainly as ammonium or nitrate.
- Microbes use an enzyme called nitrogenase to turn N₂ into ammonia.
- This uses a lot of energy.
Which microbes fix nitrogen?
- Some bacteria fix nitrogen while living free in soil or water. Certain cyanobacteria do.
- Others live with plants in symbiosis. The plant supplies sugars. The microbe supplies nitrogen.
- Legumes are in the pea and bean family. Many form root nodules.
- Bacteria called rhizobia live in the nodules.
- Alder trees partner with Frankia bacteria.
- The water fern Azolla hosts a nitrogen-fixing cyanobacterium.
- Sources: NMSU, legume nitrogen fixation and NCERT mineral nutrition.
True or False: Alfalfa, chickpea and clover can form nitrogen-fixing nodules.
How the Root Partnership Works
Nodule formation and oxygen
- Rhizobia respond to chemicals from a legume root.
- Root hairs curl around them. An infection thread carries them in.
- Root cells divide and form a nodule.
- Oxygen can damage nitrogenase, yet the bacteria need oxygen for energy.
- The protein leghaemoglobin keeps free oxygen low.
- Active nodules often look pink inside because of it.
The six plants in the UPSC question
| Plant | Fixes nitrogen? | Why |
|---|---|---|
| 1 Alfalfa | Yes | Legume with root nodules |
| 2 Amaranth | No | No such nodules |
| 3 Chickpea | Yes | Pulse crop with nodules |
| 4 Clover | Yes | Fodder legume with nodules |
| 5 Purslane (Kulfa) | No | No such nodules |
| 6 Spinach | No | No such nodules |
True or False: Plant roots perform nitrogen fixation without microbes.
Previous Year Questions
UPSC 2022 asked which are nitrogen-fixing plants.
- UPSC 2022Alfalfa, amaranth, chickpea, clover, purslane (kulfa) and spinach: which are nitrogen-fixing? The answer is (a) 1, 3 and 4 only: alfalfa, chickpea and clover. Sources: UPSC paper, Q48. View the question.
True or False: UPSC 2022 included spinach among the correct plants.
True or False: Nitrogenase converts nitrogen gas into usable nitrogen compounds.
What Fixation Does and Does Not Guarantee
- A legume uses much of its fixed nitrogen for its own growth.
- The next crop gains only what remains in roots, leaves and residues.
- Removed grain and fodder carry nitrogen away.
- Green manure releases nutrients as it decays.
- When soil already has plenty of nitrate, a legume may form fewer or less active nodules.
- An inoculant adds bacteria to seeds or soil. The strain must suit the crop, and heat or drying can kill it.
- Acid soil, waterlogging, drought and nutrient shortages reduce fixation.
- Source: NMSU, inoculating legumes.
Exam reminders
- “Nitrogen-fixing plant” means a plant-microbe partnership.
- Legumes are common hosts, not the only ones.
- Fixation does not give the next crop an instant supply of fertiliser.
Think it through: why does a nitrogen-fixing crop not hand all its nitrogen to the next crop?
The legume first uses nitrogen to build its own leaves, stems and seeds. Harvest then removes grain or fodder, with nitrogen inside. Only roots and leftover residues add nitrogen to the soil, and they release it slowly as they decay.
True or False: Nodule oxygen must be controlled because nitrogenase is oxygen-sensitive.
True or False: Fixed nitrogen always passes completely to the next crop.
True or False: Soil conditions and bacterial strain can change fixation rates.
Quick Q&A
Which enzyme?
Nitrogenase.
Which bacteria?
Rhizobia.
Oxygen guard?
Leghaemoglobin.
UPSC 2022 answer?
1, 3 and 4 only.
Non-legume host?
Alder, Azolla.
🎲 Practice: Nitrogen-Fixing Plants: Which of the Six Actually Qualify
3 verified questions from this topic.
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