The CRISPR-Cas9 gene-editing breakthrough was developed in 2012 by which two scientists?

Jennifer Doudna and Emmanuelle Charpentier. Doudna and Charpentier showed in 2012 that a two-RNA structure guides Cas9 to cut DNA. They shared the 2020 Nobel Prize in Chemistry. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

After Cas9 makes a double-strand break, which repair route is the dominant one and is error-prone?

Non-homologous end joining. NHEJ is the dominant route. It joins the ends without a template, so small insertions or deletions can appear. HDR needs a template and repairs precisely. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

Which gene-editing tool changes one DNA base without a double-strand break or a donor template?

Base editing. David Liu's lab reported base editing in 2016. Cas9 is fused to an enzyme that converts one base to another, such as C to T. The indel rate was low. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

The UK regulator MHRA authorised the first medicine that uses CRISPR on 16 November 2023. What is it called?

Casgevy. The MHRA authorised Casgevy on 16 November 2023. It was the first medicine licensed that uses CRISPR. It edits the BCL11A enhancer to raise fetal haemoglobin. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

India's first indigenous CRISPR-based gene therapy for sickle cell disease, BIRSA 101, was developed at which institute?

CSIR-IGIB. PIB says BIRSA 101 was developed at CSIR-IGIB. The technology was then transferred to the Serum Institute of India. The name honours Birsa Munda. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

Which pair of genome-edited rice varieties did ICAR announce in May 2025?

DRR Rice 100 (Kamala) and Pusa DST Rice 1. PIB says ICAR developed DRR Rice 100 (Kamala) and Pusa DST Rice 1. Samba Mahsuri and MTU 1010 are the base varieties they were built on. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

Chinese scientist He Jiankui was jailed in December 2019 for which act?

Experiments on human embryos that broke a government ban. A Chinese court jailed him for three years and fined him three million yuan. He had carried out embryo experiments to give protection against HIV. WHO notes that heritable editing alters embryos and passes to descendants. Read more: SciTech0123 — CRISPR: Gene-Editing Technology.

What does the PAM do in the Cas9 system?

Practice

Quick random quizMixed questions, start right away

History

Polity & society

Economy & geography

Science & more

Aptitude

Exams

Study

History

Polity & society

Economy & geography

Science & more

Aptitude

What does the PAM do in the Cas9 system?

Keep practicing

Beyond the answer

Leave a ReplyCancel reply

Discover more from MCQ Questions