Contents
- Cells and specialisation
- Nucleus and nucleoid
- Membrane and cytoplasm
- DNA and the nucleus
- Ribosomes and rough ER
- Protein processing and smooth ER
- Respiration and mitochondria
- Digestion and vacuoles
- Cytoskeleton and centrioles
- Plant and animal cells
- Photosynthetic pigments
- Water movement by osmosis
- Tonicity and plasmolysis
- Hooke and cork cells
- Exam applications
- 4 learner questions
- 5 understanding checks
- 8 practice MCQs
1. What is a cell?
A cell is the basic structural and functional unit of living organisms. Organisms may contain one cell or many specialised cells.
A unicellular organism, such as Amoeba, performs its life processes within one cell. A multicellular organism divides many tasks among specialised cells.
For example, muscle cells generate force, while nerve cells carry signals. Specialisation connects cell structure with the organism's needs.
Many cells contain deoxyribonucleic acid (DNA), which carries genetic information. Cell structures use that information during growth and activity.
2. What separates a nucleus from a nucleoid?
A nucleus has a surrounding membrane; a nucleoid does not. This distinction helps separate two broad types of cell organisation.
| Feature | Prokaryotic organisation | Eukaryotic organisation |
|---|---|---|
| Nuclear boundary | No membrane-bound nucleus | Membrane-bound nucleus in the typical cell |
| Genetic region | Nucleoid | Most DNA within the nucleus |
| Familiar examples | Bacteria and archaea | Animals, plants, fungi and protists |
| Ribosomes | Present | Present |
| Internal compartments | Lack the usual eukaryotic membrane-bound organelles | Include specialised membrane-bound organelles |
A bacterial chromosome occupies the nucleoid region within the cytoplasm. Its DNA is organised, rather than simply drifting without structure.
Prokaryotes still contain a cell membrane and protein-making ribosomes. Having no nucleus does not mean having no internal organisation.

3. What do the cell membrane and cytoplasm do?
The cell membrane controls exchanges between a cell and its surroundings. Selective permeability means different substances cross with different ease.
The membrane forms a boundary, but it does not isolate the cell completely. A functioning cell needs materials from outside and must remove some products.
Cytosol is the fluid portion inside the cell. Cytoplasm includes that fluid and cellular structures outside the nucleus.
Calling cytoplasm only “jelly-like fluid” leaves out those structures. In a eukaryotic cell, the nucleus is distinguished from the surrounding cytoplasm.
4. How does the nucleus help control cell activity?
The nucleus stores most of a typical eukaryotic cell's DNA. DNA instructions influence which proteins the cell makes.
A nuclear envelope separates the nucleus from the cytoplasm. Nuclear pores allow selected materials to move between these regions.
The nucleolus is a region within the nucleus involved in making ribosomal components. It is different from the bacterial nucleoid.
The nucleus does not make every cellular decision by itself. Cell activity also depends on signals, enzymes and other interacting structures. Mitochondria and chloroplasts contain some DNA of their own.
5. Where do cells make proteins?
Ribosomes join amino acids into proteins. They use instructions carried by ribonucleic acid (RNA).
Some ribosomes are free in the cytosol. Others attach to the rough endoplasmic reticulum (rough ER). The attached ribosomes give rough ER its rough appearance.
Proteins destined for secretion or particular membrane compartments often enter the rough ER pathway. Many proteins used in the cytosol are made on free ribosomes.
Therefore, rough ER is not the site of every protein's production. Ribosomes perform the protein-building step.
6. What happens after a protein enters rough ER?
The ER and Golgi apparatus cooperate in processing and transporting many proteins. Small membrane sacs called vesicles carry materials between compartments.
Follow a protein intended for secretion:
- A ribosome builds the protein into the rough ER pathway.
- The ER helps process and fold the protein.
- A transport vesicle carries the protein towards the Golgi apparatus.
- The Golgi modifies and sorts the protein.
- A vesicle delivers the protein towards its destination.
The Golgi apparatus is a processing and sorting organelle. It is not the region holding a bacterium's chromosome.
Smooth ER lacks attached ribosomes. Its functions include lipid synthesis and detoxification in certain cells. Smooth ER in liver cells is a useful detoxification example.
The ER does not have one identical role in every cell. Cell specialisation affects which functions are emphasised.
7. What do mitochondria contribute to respiration?
Mitochondria support major stages of aerobic respiration in eukaryotic cells. Their inner membranes support production of adenosine triphosphate (ATP).
ATP helps couple energy-releasing processes with cellular work. Mitochondria therefore contribute to activities such as movement and transport.
However, glucose breakdown does not occur entirely inside mitochondria. Glycolysis, the initial glucose-splitting pathway, occurs in the cytosol.
Bacteria can perform respiration without mitochondria. Consequently, “respiration occurs only in mitochondria” would be too broad.
Plant cells also contain mitochondria. Photosynthesis does not remove a plant cell's need for respiration.
8. Why do cells need lysosomes and vacuoles?
Lysosomes digest selected materials inside many animal cells. Their enzymes help break down engulfed material and worn-out cellular components.
Imagine an immune cell engulfing a bacterium. Digestive compartments can combine with the compartment containing the bacterium. Enzymes then help break down the engulfed material.
Phagocytic white blood cells provide a useful example. Not every white blood cell performs the same digestive task.
Vacuoles are membrane-bound compartments with storage and other functions. A mature plant cell often has a large central vacuole. Its water contributes to pressure that supports the cell.
Animal cells can contain smaller storage or transport compartments. They do not all follow one fixed vacuole-number pattern.
9. How do the cytoskeleton and centrioles help a cell?
The cytoskeleton is a network of protein filaments. It supports cell shape and helps organise movement within the cell.
Vesicles can move along filament tracks with the help of motor proteins. A cell's internal structures therefore do not simply float without organisation.
In many animal cells, the centrosome is a microtubule-organising centre. It usually contains a pair of centrioles.
Spindle microtubules help move chromosomes during division. However, centrioles are not required for every cell's division. Many plant cells organise division without centrioles.
10. How do plant cells differ from animal cells?
Plant cells usually have a cellulose-rich wall outside their cell membrane. Animal cells lack a cell wall.
The wall helps provide shape and support. The cell membrane still controls exchanges across the living cell boundary.
Chloroplasts carry out photosynthesis in photosynthetic plant cells and many algae. They use light energy in processes that support sugar production.

Not every plant cell contains chloroplasts. A non-photosynthetic root cell is a useful contrast with a photosynthetic leaf cell.
Plant and animal cells share many structures, including ribosomes and mitochondria. A plant cell should not be described as having only chloroplasts for energy-related processes.
11. Which pigments capture light in photosynthesis?
Chlorophylls and carotenoids help photosynthetic cells handle light energy. Different pigments absorb different parts of the light spectrum.
Carotenoids contribute to light harvesting and protection against excess light energy. Excess energy can damage photosynthetic machinery if it is not safely managed.
In plant photosystems, special chlorophyll a molecules carry out the reaction-centre photochemistry. Carotenoids assist the process, but they are not the reaction-centre chlorophyll pair.
Therefore, helping absorb light differs from performing the reaction centre's particular role.
12. What is osmosis?
Osmosis is the net movement of water across a selectively permeable membrane. A difference in effective solute concentration can drive that movement.
In a simple model, consider water and a solute that cannot cross the membrane. Water moves towards the side with more of that dissolved solute.
Individual water molecules still cross in both directions. Net movement describes the difference between those opposing flows.
Osmosis is a form of passive transport. The cell does not directly spend ATP to move water through osmosis.
13. How do hypotonic, isotonic and hypertonic solutions affect cells?
Tonicity describes how a surrounding solution affects cell water balance. The comparison depends mainly on solutes that do not freely cross the membrane.
| External solution | Net water movement in the simple model | Typical animal-cell effect |
|---|---|---|
| Hypotonic | Into the cell | Swelling; possible bursting |
| Isotonic | No sustained net movement | No net volume change from osmosis |
| Hypertonic | Out of the cell | Shrinking |
Work through a comparison
Imagine an animal cell placed in a solution with more non-penetrating solute than its cytosol. The outside solution is hypertonic relative to the cell. Water leaves the cell, and its volume falls.
Reverse the comparison. Less non-penetrating solute outside makes the surrounding solution hypotonic. Water enters, and the animal cell swells.
An isotonic solution does not stop water molecules moving. Movement in both directions continues without sustained net volume change.
What changes in a plant cell?
The wall resists expansion as water enters. A hypotonic environment can make the cell turgid, meaning firm from internal pressure.
In a sufficiently hypertonic environment, water loss can cause plasmolysis. The cell's living contents shrink, pulling the membrane away from the wall. The wall itself does not shrink away from the membrane.
Net volume change is 0 in the simple isotonic model.
14. What did Robert Hooke observe in 1665?
Robert Hooke used the word “cells” for compartments he observed in cork. His illustrations appeared in Micrographia in 1665.
The cork compartments were bounded by the walls of dead plant cells. Hooke was not viewing living cellular machinery inside those compartments.
Hooke used a compound microscope. The observation introduced a name that later acquired a broader biological meaning.
15. How do these ideas appear in exam questions?
Start with the structure, then connect its function to the question's situation. A familiar organelle nickname does not replace that reasoning.
The Combined Defence Services (CDS) I 2026 General Knowledge (GK) paper includes these baseline examples:
- Question 1: a non-membrane-bound bacterial DNA region is the nucleoid.
- Question 4: lysosomes help digest material engulfed by phagocytic white blood cells.
- Question 5: carotenoids assist photosynthesis but do not form the reaction-centre chlorophyll pair.
- Question 9: mitochondria support respiration; cytoskeleton supports shape; smooth ER supports detoxification; vacuoles provide storage.
The National Defence Academy and Naval Academy (NDA-NA) II 2016 paper includes Question 99. It asks about bacterial genetic material. “Cytoplasm” describes its broad location, while “nucleoid” names the particular region.
The answer therefore depends on what the question asks you to identify. A location and a named organelle are not interchangeable.
Questions and answers
Why does rough ER look rough?
Ribosomes attach to its surface. Smooth ER lacks those attached ribosomes.
Can a plant cell contain both chloroplasts and mitochondria?
Yes. Photosynthetic plant cells can contain both. Chloroplasts support photosynthesis, while mitochondria support aerobic respiration.
Why can an isotonic cell still exchange water?
Water molecules move across the membrane in both directions. Balanced flows produce no sustained net volume change.
Why is a nucleolus different from a nucleoid?
A nucleolus lies within a eukaryotic nucleus and helps make ribosome components. A nucleoid is a prokaryotic DNA region without a nuclear envelope.
Check your understanding
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